- gene of interest is first cut out and removed from a cell
- a bacterial vector is also cut using the same enzyme to create two sticky ends
- the sticky ends is used to connect the original vector to the new gene from the original host
- newly formed vectors, recombinant DNA, (containing ampicillin resistance genes) are placed with the bacteria E. Coli, and some E. Coli cells will pick up the new vector through the process of transformation
- the bacteria cells will then be placed in a container with yeast as their nutrient and ampicillin
- bacteria cells without ampicillin resistance genes would be killed while the one that received the new vector would grow slowly
- the surviving "desired" bacteria cells, those with the recombinant DNA, are then being cloned
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